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KMID : 0368420020450040212
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Journal of Plant Biology
2002 Volume.45 No. 4 p.212 ~ p.218
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Isolation and Characterization of Drought-Induced cDNA Clones from Hot Pepper(Capsicum annuum)
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Choi, Kyu Ha
Hong, Choo Bong/Kim, Woo Taek
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Abstract
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Drought is a major environmental factor that limits plant growth and productivity. After constructing a cDNA library from progressively drought-stressed hot pepper plants (Capsicum annuum), we differentially screened it to isolate cDNA clones whose expressions were drought-inducible. We named these clones CaDSi-1, 5, 7, 11, and 12. RNA blot analyses revealed that all transcripts of these genes were induced strongly or slightly during progressive drought stress. Their deduced amino-acid sequences showed high homologies with such proteins as thioredoxin m, glycine-rich protein, nonspecific lipid transfer protein, oxygen-evolving enhancer protein 3, and auxin-repressed protein. In other plant species, those proteins are induced by pathogens and water or salt stresses, and are predicted to be involved in drought tolerance. The data suggest that these five CaDSi gene products have possible protective roles against drought stress in hot pepper.
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